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Autoimmune Encephalitis Antibodies (LGI1, CASPR2, NMDA, DPPX, AMPA1/2, GABA B)

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Test Background

This test is performed by IIF on transfected fixed cells.

The autoimmune encephalitidies are immune-mediated brain disorders that often present rapidly (The below table lists the most common autoantibodies found in autoimmune encephalitis in descending order of frequency, those in bold are included in the autoimmune encephalitis screen.

Table adapted from Uy CE, Binks S, Irani SR. Autoimmune encephalitis: clinical spectrum and management. Practical Neurology 2021;21:412-423

Antibodies should be measured in CSF and serum. Any positive serum result should always be confirmed in CSF to reduce the risk of misdiagnosis (Dalmau J, Graus F. JAMA Neurol. 2023 Jan 1;80(1):12-14.)

This test detects antibodies to a panel of neuronal cell surface antigens including the glutamate receptors NMDAR and AMPA1/2, GABAB receptors, DPPX and the voltage gated potassium channel VGKC-associated proteins LGI1 and CASPR2. See also Neuronal (paraneoplastic) antibody screen, MOG, Aquaporin, glycine receptor antibodies.

The below table lists the most common autoantibodies found in autoimmune encephalitis in descending order of frequency, those in bold are included in the autoimmune encephalitis screen.

Table adapted from Uy CE, Binks S, Irani SR. Autoimmune encephalitis: clinical spectrum and management. Practical Neurology 2021;21:412-423

Antibodies should be measured in CSF and serum. Any positive serum result should always be confirmed in CSF to reduce the risk of misdiagnosis (Dalmau J, Graus F. JAMA Neurol. 2023 Jan 1;80(1):12-14.)

This test detects antibodies to a panel of neuronal cell surface antigens including the glutamate receptors NMDAR and AMPA1/2, GABAB receptors, DPPX and the voltage gated potassium channel VGKC-associated proteins LGI1 and CASPR2. See also Neuronal (paraneoplastic) antibody screen, MOG, Aquaporin, glycine receptor antibodies.

Clinical Indications

The differential diagnosis of encephalitis when alternative causes (infection, neoplasm, etc.) have been excluded.

Reference Range

Negative

Sample volume
5-10 mL blood (2 mL serum), 0.5ml CSF
Turnaround time
14 days, for urgent requests please liaise with the consultant clinical scientist
Sample & Container Required
CSF & Serum RST (rust top or SST yellow top)
Notes

Referred Test. Not currently performed in house.

Referral Laboratory: University Hospital, Birmingham.

Record last updated
September 4, 2025

Sample Container

5ml RST Vacutainers (Rust top)

Sample Container

5ml SST Vacutainers (Gold / Yellow top)

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Test Results

Yes, we have a Pathology User Guide

Yes. Please email your request to Imperial.nwlpcustomerservice@nhs.net

Results are communicated to clinical staff and are normally not communicated direct to patients either on telephone requests or in written report. If you need to contact us please click here to view the contact details page

Please contact the Customer Service Team Monday to Friday from 8:30am – 6:00pm on 0203 313 5353. Alternatively, see the Pathology User Guide for how to contact the relevant department. If advice is not urgent you can also email your query to Imperial.nwlpcustomerservice@nhs.net

Additional tests may be added by discussion with the relevant laboratory. The time limit for adding additional tests to a sample already received in the laboratory will depend on the type of sample and the department it was sent to.

Information on the repertoire of tests performed by pathology, including reference ranges, turnaround times and specimen requirements, can be found in the test directory.

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See the following UKAS ref numbers:

  • Clinical Biochemistry Ref: 8673
  • Haematology and Blood Transfusion Ref: 8674
  • Infection & Immunity Sciences (Including Immunology, Virology, Microbiology and Histocompatibility and Immunogenetics ) Ref: 8659
  • Cellular Pathology (Including Histopathology, Cytology and Molecular Pathology) Ref: 9615

If you require copies of our certificate of accreditation and associated documentation please contact Imperial.nwlpcustomerservice@nhs.net.

UKAS requires the laboratories to be accredited for a particular repertoire/scope (ISO15189), any changes or additions to repertoire require assessment by UKAS (extension to scope).

The BD Hospital Tube GuideBD GP Tube Guide and BD Paediatric Tube Guide provide visual references to the correct tube types and collection instructions.

Home testing

INSTRUCTION SHEET FOR BLOOD SAMPLE COLLECTION 

We also have two instruction videos available (one with subtitles and one without) – please click on the links to view the videos on Google.

VIDEO ON BLOOD SAMPLE COLLECTION (without subtitles)

VIDEO ON BLOOD SAMPLE COLLECTION (with subtitles)

Our accreditations

Upholding excellence in diagnostic standards

Our laboratories are accredited by the UK Accreditation Service (UKAS) against the international standard ISO15189:2022. UKAS is the accreditation body for the UK that assesses medical laboratories.

Find out more about our accreditations on the UKAS website.

UKAS website
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