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Calprotectin faecal (Faecal, Calprotectin)

Test Background

Calprotectin is a complex of two proteins S100A8 & S100A9, which belong to the S100 family of calcium binding proteins. They are highly expressed in resting neutrophils, keratinocytes, infiltrating tissue macrophages and on epithelial cells active in inflammatory disease. As calprotectin comprises 60% of the soluble protein in neutrophils, intestinal inflammation results in elevated concentrations being present in the stool. Faecal calprotectin correlates with the number of neutrophil granulocytes in the intestinal lumen and is thus elevated in inflammatory bowel diseases (IBD) such as Crohn's disease and ulcerative colitis, and to a smaller and variable extent in other disorders such as neoplasia and polyps.

Faecal Calprotectin measurement is a robust, non-invasive test, shown to be the most sensitive and most specific test for differentiating IBD from IBS (irritable bowel syndrome) and other functional disorders. The concentration of faecal calprotectin present in the stool also correlates with disease activity and is therefore a useful monitoring tool able to predict relapses in IBD.

Clinical Indications

Faecal calprotectin is typically requested to determine the possible present of gastrointestinal inflammation; often specifically to distinguish between inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS) to exclude the requirement for endoscopy.

Symptoms of IBD are variable but may include diarrhoea, abdominal pain, presence of blood in the stool, fever, weakness and weight loss. In known cases of IBD, monitoring of faecal calprotectin can be used to determine the presence of a flare up in disease activity and/or remission.

Reference Range

 

< 50 mg/Kg If no red flags or other abnormal test results, manage as IBS.
50-150 mg/Kg Repeat test after 4 weeks. If this is a repeat result 50 – 150 µg/g and symptoms persist, consider refer to Gastroenterology.
>150 mg/Kg Higher probability of organic pathology than IBS. Refer to Gastroenterology/Direct to test as appropriate.

 

Sample volume
10g of formed stool sample (1g minimum).
Turnaround time
1 week
Sample & Container Required
A formed stool sample should be collected in a sterile 25 ml universal container (1g minimum, 10g optimal). Please include any known clinical symptoms and drug history to aid clinical interpretation of results.
Transport Storage
Samples are stable for up to 1 week if stored at 2°C - 8°C (samples may be stored for longer intervals at -20°C, however avoid freeze thaw cycles). Please send to the lab as soon as possible.

Record last updated
September 15, 2025

Sample Container

30ml Universal Container with Spoon – Stool (Blue top)

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Test Results

Yes, we have a Pathology User Guide

Yes. Please email your request to Imperial.nwlpcustomerservice@nhs.net

Results are communicated to clinical staff and are normally not communicated direct to patients either on telephone requests or in written report. If you need to contact us please click here to view the contact details page

Please contact the Customer Service Team Monday to Friday from 8:30am – 6:00pm on 0203 313 5353. Alternatively, see the Pathology User Guide for how to contact the relevant department. If advice is not urgent you can also email your query to Imperial.nwlpcustomerservice@nhs.net

Additional tests may be added by discussion with the relevant laboratory. The time limit for adding additional tests to a sample already received in the laboratory will depend on the type of sample and the department it was sent to.

Information on the repertoire of tests performed by pathology, including reference ranges, turnaround times and specimen requirements, can be found in the test directory.

NWLP operates UKAS accredited medical laboratories as per the references below. The current scope of accreditation for all NWLP’s laboratories can be found on the UKAS website.

See the following UKAS ref numbers:

  • Clinical Biochemistry Ref: 8673
  • Haematology and Blood Transfusion Ref: 8674
  • Infection & Immunity Sciences (Including Immunology, Virology, Microbiology and Histocompatibility and Immunogenetics ) Ref: 8659
  • Cellular Pathology (Including Histopathology, Cytology and Molecular Pathology) Ref: 9615

If you require copies of our certificate of accreditation and associated documentation please contact Imperial.nwlpcustomerservice@nhs.net.

UKAS requires the laboratories to be accredited for a particular repertoire/scope (ISO15189), any changes or additions to repertoire require assessment by UKAS (extension to scope).

The BD Hospital Tube GuideBD GP Tube Guide and BD Paediatric Tube Guide provide visual references to the correct tube types and collection instructions.

Home testing

INSTRUCTION SHEET FOR BLOOD SAMPLE COLLECTION 

We also have two instruction videos available (one with subtitles and one without) – please click on the links to view the videos on Google.

VIDEO ON BLOOD SAMPLE COLLECTION (without subtitles)

VIDEO ON BLOOD SAMPLE COLLECTION (with subtitles)

Our accreditations

Upholding excellence in diagnostic standards

Our laboratories are accredited by the UK Accreditation Service (UKAS) against the international standard ISO15189:2022. UKAS is the accreditation body for the UK that assesses medical laboratories.

Find out more about our accreditations on the UKAS website.

UKAS website
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