Delivering Science Supporting Healthcare

Complement C1 esterase inhibitor levels and function (C1 inhibitor)

Service
Test Background

C1 esterase inhibitor regulates the classical complement pathway, the coagulation and the kinin systems. Genetic mutations in the SERPING1 gene leading to C1 inhibitor deficiency cause hereditary angioedema (HAE). Patients present with bradykinin mediated acute deep tissue oedema, typically of the face, larynx or abdomen that can be life threatening if respiratory obstruction occurs. Urticaria is not a feature.

85% of HAE patients have low antigenic C1 esterase inhibitor levels (type I HAE), a smaller proportion have normal levels but a non-functioning protein (HAE 2). During acute attacks the complement C4 level is low and C3 level is normal.

Acquired C1 inhibitor deficiency (AAE) caused by complement activation or autoantibodies to C1 inhibitor is clinically indistinguishable from HAE but presents in older patients and is usually associated with lymphoproliferative or autoimmune disease. Statins, ACE inhibitors and some antipsychotic drugs can also cause angioedema without urticaria. Patients with AAE can be distinguished from HAE by measuring C1q levels.

Rarer forms of HAE can be caused by mutations in other members of the kallikrein/kinin pathway (FactorXII, plasminogen, angiopoietin 1, kininogen 1, myoferlin), these patients have normal C1 inhibitor levels.

C1 inhibitor levels are measured by turbidimetry and C1 inhibitor function is measured by chromogenic assay.

Clinical Indications

Recurrent angioedema in the absence of urticaria
Family history of HAE

Reference Range

C1 inhibitor level = 0.22-0.38 g/L
C1 inhibitor function = 0.7-1.3 IU/mL (70-130% activity compared to the reference standard)
Reference ranges are manufacturer derived and were verified in house

Sample volume
5-10 mL blood (1 mL serum)
Turnaround time
21 days
Sample & Container Required
Serum (rust top RST tube)
Transport Storage
For external users, separate and freeze immediately. Transport frozen. For internal users, transport to Charing Cross Hospital Immunology ASAP

Record last updated
August 15, 2025

Sample Container

5ml RST Vacutainers (Rust top)

Search test database

Test Results

Yes, we have a Pathology User Guide

Yes. Please email your request to Imperial.nwlpcustomerservice@nhs.net

Results are communicated to clinical staff and are normally not communicated direct to patients either on telephone requests or in written report. If you need to contact us please click here to view the contact details page

Please contact the Customer Service Team Monday to Friday from 8:30am – 6:00pm on 0203 313 5353. Alternatively, see the Pathology User Guide for how to contact the relevant department. If advice is not urgent you can also email your query to Imperial.nwlpcustomerservice@nhs.net

Additional tests may be added by discussion with the relevant laboratory. The time limit for adding additional tests to a sample already received in the laboratory will depend on the type of sample and the department it was sent to.

Information on the repertoire of tests performed by pathology, including reference ranges, turnaround times and specimen requirements, can be found in the test directory.

NWLP operates UKAS accredited medical laboratories as per the references below. The current scope of accreditation for all NWLP’s laboratories can be found on the UKAS website.

See the following UKAS ref numbers:

  • Clinical Biochemistry Ref: 8673
  • Haematology and Blood Transfusion Ref: 8674
  • Infection & Immunity Sciences (Including Immunology, Virology, Microbiology and Histocompatibility and Immunogenetics ) Ref: 8659
  • Cellular Pathology (Including Histopathology, Cytology and Molecular Pathology) Ref: 9615

If you require copies of our certificate of accreditation and associated documentation please contact Imperial.nwlpcustomerservice@nhs.net.

UKAS requires the laboratories to be accredited for a particular repertoire/scope (ISO15189), any changes or additions to repertoire require assessment by UKAS (extension to scope).

The BD Hospital Tube GuideBD GP Tube Guide and BD Paediatric Tube Guide provide visual references to the correct tube types and collection instructions.

Home testing

INSTRUCTION SHEET FOR BLOOD SAMPLE COLLECTION 

We also have two instruction videos available (one with subtitles and one without) – please click on the links to view the videos on Google.

VIDEO ON BLOOD SAMPLE COLLECTION (without subtitles)

VIDEO ON BLOOD SAMPLE COLLECTION (with subtitles)

Our accreditations

Upholding excellence in diagnostic standards

Our laboratories are accredited by the UK Accreditation Service (UKAS) against the international standard ISO15189:2022. UKAS is the accreditation body for the UK that assesses medical laboratories.

Find out more about our accreditations on the UKAS website.

UKAS website
Man in the lab handling a sample