Delivering Science Supporting Healthcare

Haemosiderin (urinary)

Service
Test Background

Haemoglobinuria and haemoglobinaemia occur when haemoglobin is released into the plasma in greater amounts than can be bound to haptoglobins. Haemoglobin bound to haptoglobin is unable to cross the glomerular membrane, but free haemoglobin is sufficiently small to be excreted in the urine. In acute intravascular haemolysis, haemoglobin appears in the urine more or less intact. In chronic haemoglobinuria, some of the pigment is taken up by the kidney and degraded and haemosiderin granules are passed into the urine. The presence of haemosiderin in the urine indicates the recent or chronic release of free haemoglobin into the circulating plasma, and the depletion of haemopexin and haptoglobin.

Haemosiderin is usually attributable to intravascular haemolysis, but may originate from conditions such as haemorrhagic pancreatitis, in which breakdown of blood cells occurs in the peritoneal cavity.

Clinical Indications

Urinary haemosiderin may aid diagnosis of haemolytic anaemia associated with intravascular haemolysis, eg. haemolytic transfusion reactions, cold haemagglutinin disease, paroxysmal nocturnal haemoglobinuria (PNH), thalassaemia major, sickle cell disease, trauma due to replacement mechanical heart valves, and drug-induced oxidative stress (with/without G6PD/ PK deficiency)
Diagnosis and assessment of iron overload conditions eg. post-transfusion, hereditary haemochromatosis, etc.

Sample volume
5.0 mL
Turnaround time
5 Days
Sample & Container Required
Early morning urine (EMU) in universal container

Record last updated
August 20, 2025

Sample Container

150ml Universal Container for Urine TB (Silver top)

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Test Results

Yes, we have a Pathology User Guide

Yes. Please email your request to Imperial.nwlpcustomerservice@nhs.net

Results are communicated to clinical staff and are normally not communicated direct to patients either on telephone requests or in written report. If you need to contact us please click here to view the contact details page

Please contact the Customer Service Team Monday to Friday from 8:30am – 6:00pm on 0203 313 5353. Alternatively, see the Pathology User Guide for how to contact the relevant department. If advice is not urgent you can also email your query to Imperial.nwlpcustomerservice@nhs.net

Additional tests may be added by discussion with the relevant laboratory. The time limit for adding additional tests to a sample already received in the laboratory will depend on the type of sample and the department it was sent to.

Information on the repertoire of tests performed by pathology, including reference ranges, turnaround times and specimen requirements, can be found in the test directory.

NWLP operates UKAS accredited medical laboratories as per the references below. The current scope of accreditation for all NWLP’s laboratories can be found on the UKAS website.

UKAS accredited medical laboratory/medical diagnostic service:

  • Clinical Biochemistry No. 8673
  • Haematology and Blood Transfusion No. 8674
  • Infection & Immunity Sciences (Including Immunology, Virology, Microbiology and Histocompatibility and Immunogenetics ) No. 8659
  • Cellular Pathology (Including Histopathology, Cytology and Molecular Pathology) No. 9615

If you require copies of our certificate of accreditation and associated documentation please contact Imperial.nwlpcustomerservice@nhs.net.

UKAS requires the laboratories to be accredited for a particular repertoire/scope (ISO15189), any changes or additions to repertoire require assessment by UKAS (extension to scope).

The BD Hospital Tube GuideBD GP Tube Guide and BD Paediatric Tube Guide provide visual references to the correct tube types and collection instructions.

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INSTRUCTION SHEET FOR BLOOD SAMPLE COLLECTION 

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VIDEO ON BLOOD SAMPLE COLLECTION (without subtitles)

VIDEO ON BLOOD SAMPLE COLLECTION (with subtitles)

Our accreditations

Upholding excellence in diagnostic standards

Our laboratories are accredited by the UK Accreditation Service (UKAS) against the international standard ISO15189:2022. UKAS is the accreditation body for the UK that assesses medical laboratories.

Find out more about our accreditations on the UKAS website.

UKAS website
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