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HLA typing -PCR-SSO (low/medium resolution)

Test Background

HLA typing at medium/low resolution typing for HLA-DQA1, -DQB1, -DRB345 is performed using PCR-SSO. This technique is primarily used for the HLA typing of solid organ patients and donors.

Clinical Indications

HLA typing at HLA-DQA1, -DQB1, -DRB345 alongside high resolution NGS HLA typing allows for a full HLA typing result to be achieved for solid organ patients and donors. HLA typing alongside HLA antibody detection techniques allows full HLA compatibility to be determined for solid organ patients and their prospective donors.

Reference Range

An HLA tissue typing report will be provided.

Sample volume
Minimum - 4 mL
Turnaround time
Renal/ pancreas patients and donors -30 days
Sample Container
4mL Whole Blood – EDTA (lavender top) 2x Buccal swabs (swab kit to be requested from laboratory for required HSCT patients and donors)
Transport Storage
Samples must be sent by courier.
Notes

Samples must be sent via first class post or courier.

The laboratory is accredited for this test by the European Federation of Immunogenetics (EFI) and UKAS for HLA typing using EDTA samples and buccal swabs.

 

Record last updated
September 2, 2025

Sample Container

4ml EDTA Vacutainers (Lavender top)

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Test Results

Yes, we have a Pathology User Guide

Yes. Please email your request to Imperial.nwlpcustomerservice@nhs.net

Results are communicated to clinical staff and are normally not communicated direct to patients either on telephone requests or in written report. If you need to contact us please click here to view the contact details page

Please contact the Customer Service Team Monday to Friday from 8:30am – 6:00pm on 0203 313 5353. Alternatively, see the Pathology User Guide for how to contact the relevant department. If advice is not urgent you can also email your query to Imperial.nwlpcustomerservice@nhs.net

Additional tests may be added by discussion with the relevant laboratory. The time limit for adding additional tests to a sample already received in the laboratory will depend on the type of sample and the department it was sent to.

Information on the repertoire of tests performed by pathology, including reference ranges, turnaround times and specimen requirements, can be found in the test directory.

NWLP operates UKAS accredited medical laboratories as per the references below. The current scope of accreditation for all NWLP’s laboratories can be found on the UKAS website.

See the following UKAS ref numbers:

  • Clinical Biochemistry Ref: 8673
  • Haematology and Blood Transfusion Ref: 8674
  • Infection & Immunity Sciences (Including Immunology, Virology, Microbiology and Histocompatibility and Immunogenetics ) Ref: 8659
  • Cellular Pathology (Including Histopathology, Cytology and Molecular Pathology) Ref: 9615

If you require copies of our certificate of accreditation and associated documentation please contact Imperial.nwlpcustomerservice@nhs.net.

UKAS requires the laboratories to be accredited for a particular repertoire/scope (ISO15189), any changes or additions to repertoire require assessment by UKAS (extension to scope).

The BD Hospital Tube GuideBD GP Tube Guide and BD Paediatric Tube Guide provide visual references to the correct tube types and collection instructions.

Home testing

INSTRUCTION SHEET FOR BLOOD SAMPLE COLLECTION 

We also have two instruction videos available (one with subtitles and one without) – please click on the links to view the videos on Google.

VIDEO ON BLOOD SAMPLE COLLECTION (without subtitles)

VIDEO ON BLOOD SAMPLE COLLECTION (with subtitles)

Our accreditations

Upholding excellence in diagnostic standards

Our laboratories are accredited by the UK Accreditation Service (UKAS) against the international standard ISO15189:2022. UKAS is the accreditation body for the UK that assesses medical laboratories.

Find out more about our accreditations on the UKAS website.

UKAS website
Man in the lab handling a sample