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Extractable nuclear (ENA) antibodies

Category Immunology
Test background

This test detects antibodies against a group of antigens in the nucleus and cytoplasm. ENA antibodies are useful in classifying clinical subsets of connective tissue diseases. The screen includes: anti-Ro52 and anti-Ro60 (SS-A), anti-La (SS-B) antibodies seen in Sjögren’s syndrome and SLE; anti-Sm antibody specific for SLE; anti-ribonucleoprotein (RNP) antibodies seen in mixed connective tissue disease; anti-Jo-1 antibody seen in myositis and anti-synthetase syndrome; anti-Scl-70 (topoisomerase-1) antibody seen in scleroderma; anti-centromere B antibodies seen in limited (cutaneous) scleroderma and ribosomal P antibodies associated with neuropsychiatric lupus.

Clinical Indications

Suspected autoimmune-mediated connective tissue disorders ‘Speckled’ pattern observed on antinuclear antibody immunofluorescence

Reference range


Sample & container required RST (rust top)
Sample volume 5-10 mL (1 mL serum)
Turnaround time 10 days screen

This test is currently unaccredited due to a recent change in platform/assay.