Extractable nuclear (ENA) antibodies
| Category | Immunology |
|---|---|
| Test background |
This test detects antibodies against a group of antigens in the nucleus and cytoplasm. ENA antibodies are useful in classifying clinical subsets of connective tissue diseases. The screen includes: anti-Ro52 and anti-Ro60 (SS-A), anti-La (SS-B) antibodies seen in Sjögren’s syndrome and SLE; anti-Sm antibody specific for SLE; anti-ribonucleoprotein (RNP) antibodies seen in mixed connective tissue disease; anti-Jo-1 antibody seen in myositis and anti-synthetase syndrome; anti-Scl-70 (topoisomerase-1) antibody seen in scleroderma; anti-centromere B antibodies seen in limited (cutaneous) scleroderma and ribosomal P antibodies associated with neuropsychiatric lupus. |
| Clinical Indications |
Suspected autoimmune-mediated connective tissue disorders ‘Speckled’ pattern observed on antinuclear antibody immunofluorescence |
| Reference range | Negative. |
| Sample & container required | RST (rust top) |
| Sample volume | 5-10 mL (1 mL serum) |
| Turnaround time | 10 days screen |
| Notes | This test is currently unaccredited due to a recent change in platform/assay. |