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HLA typing -PCR-SSP (low/medium resolution)

Category Immunology >> Histocompatibility and Immunogenetics
Test background

HLA typing at medium/low resolution typing for HLA-A, -B, -C, -DRB1, -DQA1, -DQB1, -DPA1 and –DPB1 is performed using PCR-SSP. This technique allows quick turn-around of HLA typing results primarily for deceased donor HLA typing

It can also be used for rapid low/medium resolution HLA typing at the request of the clinical team.

Clinical Indications

This resolution of HLA typing is of particular importance in the provision of deceased donor HLA typing in support on UK national deceased donor transplant programme. Due to the nature of deceased donor typing it is important that HLA typing result is achieved within a short time frame.

Reference range

An HLA tissue typing report will be provided.

Sample volume 4 mL
Sample container 4mL Whole Blood – EDTA (lavender top)
Transport storage Samples must be sent by courier.
Turnaround time < 4 hrs for deceased donor HLA typing

The laboratory is accredited for this test by the European Federation of Immunogenetics (EFI) and UKAS for HLA typing using EDTA samples.