Skip to main content

Login to North West London Pathology – consumables service

If you need a new account or you have a query about your order, please telephone the pathology call centre on 0203 313 5353.

If you have forgotten your password you can reset this yourself. Please click here if you have forgotten your password.

Results Line and Enquiries

0203 313 5353

B-cell clonality (IGH)

Category Specialist Integrated Haematological Malignancy Diagnostic Service (SIHMDS) >> Molecular Pathology
Test background

Owing to the huge diversity in Ig/TCR rearrangements, the diversity of different Ig or TCR molecules is estimated to be in the order of 1012 (10 000 000 000 000).

B-cell clonality studies can help to discriminate between malignant and reactive lymphoproliferations in patients with suspected lymphoproliferative disorder. Multiplex PCR is performed using BIOMED-2 primers followed by fragment analysis to detect presence of clonal rearrangements in Ig heavy chain gene (IGH(V-J) and Ig kappa light chain gene (IGK and IGKde).

Click here to download the SIHMDS request form

Clinical Indications

For diagnostic testing in lymphoid malignancies such as lymphomas.

Sample & container required A minimum of 1ml peripheral blood or bone marrow in EDTA (lavender/purple top). Please note that samples must be received within 72 hours of collection for lymphocyte separation to be performed. FFPE tissue curls, uncoated/uncharged slides. Minimal tumour content of 5% is required for clonality studies. The tumour content and the amount of tissue to be cut must be assessed by a histopathologist prior to sending the sample.
Sample volume 1 mL blood OR bone marrow
Sample collection

Please ensure the sample is sent to the lab ASAP after collection as it must be received within 72 hours.

Turnaround time 14 calendar days