B-cell clonality (IGH)
|Specialist Integrated Haematological Malignancy Diagnostic Service (SIHMDS) >> Molecular Pathology
Owing to the huge diversity in Ig/TCR rearrangements, the diversity of different Ig or TCR molecules is estimated to be in the order of 1012 (10 000 000 000 000).
B-cell clonality studies can help to discriminate between malignant and reactive lymphoproliferations in patients with suspected lymphoproliferative disorder. Multiplex PCR is performed using BIOMED-2 primers followed by fragment analysis to detect presence of clonal rearrangements in Ig heavy chain gene (IGH(V-J) and Ig kappa light chain gene (IGK and IGKde).
For diagnostic testing in lymphoid malignancies such as lymphomas.
|Sample & container required
|A minimum of 1ml peripheral blood or bone marrow in EDTA (lavender/purple top). Please note that samples must be received within 72 hours of collection for lymphocyte separation to be performed. FFPE tissue curls, uncoated/uncharged slides. Minimal tumour content of 5% is required for clonality studies. The tumour content and the amount of tissue to be cut must be assessed by a histopathologist prior to sending the sample.
|1 mL blood OR bone marrow
Please ensure the sample is sent to the lab ASAP after collection as it must be received within 72 hours.
|14 calendar days