Dye binding
| Category | Haematology |
|---|---|
| Test background |
The fluorescent dye EMA (eosin-5-maleimide ) is used to label band 3 protein, which indirectly interacts with the spectrin-based cytoskeleton and membrane lipid bilayer in red blood cells. Deficiency or abnormality of band 3 is seen in hereditary spherocytosis red cells, and has also been observed in cases of Southeast Asian ovalocytosis, congenital dyserythropoietic anaemia type II, and the cryohydrocytosis variants of stomatocytosis. |
| Clinical Indications |
Unexplained haemolysis Spherocytosis Suspected hereditary spherocytosis |
| Reference range | 0.83-1.17 (ratio) |
| Sample & container required | 4 mL EDTA (lavender top) adults 0.5 mL EDTA (lavender top) paediatrics |
| Sample volume | 0.5 mL adult tube 0.2 mL paediatric tube |
| Turnaround time | Within 4 working days |
| Notes | Please contact the laboratory on 0203 312 1084 (or ext 21084) to make prior arrangements before sending samples. |