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Dye binding

Category Haematology
Test background

The fluorescent dye EMA (eosin-5-maleimide ) is used to label band 3 protein, which indirectly interacts with the spectrin-based cytoskeleton and membrane lipid bilayer in red blood cells.

Deficiency or abnormality of band 3 is seen in hereditary spherocytosis red cells, and has also been observed in cases of Southeast Asian ovalocytosis, congenital dyserythropoietic anaemia type II, and the cryohydrocytosis variants of stomatocytosis.

Clinicial Indications

Unexplained haemolysis Spherocytosis Suspected hereditary spherocytosis

Reference range

0.83-1.17 (ratio)

Sample & container required 4 mL EDTA (lavender top) adults 0.5 mL EDTA (lavender top) paediatrics
Sample volume 0.5 mL adult tube 0.2 mL paediatric tube
Turnaround time Within 4 working days