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Electron Microscopy

Category Electron Microscopy
Clinicial Indications

Ultrastructural features of cells within a tissue sample is examined to supplement and guide the clinical team to adopt the most appropriate treatment pathway for each individual patient. The EM Unit provides a service for the West-London Renal and Transplant Unit based at Hammersmith Hospital; the results of the ultrastructural examinations by Dr’s Jill Moss and Linda Moran supplement the light microscopy including special stains and immunocytochemistry to provide a diagnostic renal service co-ordinated by the Consultant Renal Histopathologists Professor Terry Cook ([email protected]) and Dr Candice Roufosse ([email protected])

Sample & container required See Notes
Turnaround time The EM Unit aims to have 95% of renal native biopsies ultrastructurally reported within 7 calendar days, although this may not always be achievable due to the complex interpretive nature of the EM specimens
Notes

At times, it may be necessary to refer work to other laboratories or consultants. This requirement is assessed on a case-by-case basis.

Sample Required

  • Renal core biopsies – a core biopsy needs to be sent to the EM Unit (Histopathology Department) in an adequately sized container, fully labelled with patient demographics.  All core biopsies require sufficient 3 % glutaraldehyde in 0.1M sodium cacodylate buffer to cover the specimen (Fixative available in advance on request)
  • Other surgical specimens – small tissue samples (no greater than 1 mm in one dimension) needs to be sent to the EM Unit (Histopathology Department) in an adequately sized container, fully labelled with patient demographics.  All biopsies require sufficient 3 % glutaraldehyde in 0.1M sodium cacodylate buffer to cover the specimen (Fixative available in advance on request)
  • Wax blocks – wax blocks for reprocessing for ultrastructural examination should be marked with the sending laboratory’s accession number. Ensure the blocks are wrapped in protective material to prevent damage in transit

Enquiries (to Clinical Scientists)

Head of Electron Microscopy Unit:
Dr Jill Moss PhD FRCPath
Email: [email protected] or telephone: 0203 311 7147

Deputy:
Dr Linda Moran PhD
Email: [email protected] or telephone: 0203 311 7147