ENA (Extractable nuclear antigen) antibodies
| Category | Immunology |
|---|---|
| Test background |
This test detects the most common nuclear and cytoplasmic IgG antibodies found in connective tissue disease. ENA are useful in classifying clinical subsets of connective tissue diseases. The ENA panel is tested following a positive ANA or CTD screen by a multiplex magnetic bead based immunoassay and includes Ro52, Ro60, SSB/La, Sm, RNP, Ribosomal P, Jo-1, Scl-70 (topoisomerase 1) and centromere B antibodies. 
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| Clinical Indications |
Suspected autoimmune-mediated connective tissue disorders |
| Reference range | Negative <1.0 antibody index (range derived by manufacturer and verified in house) |
| Sample & container required | Serum (RST rust top) |
| Sample volume | 5-10 mL blood (1 mL serum) |
| Turnaround time | 10 days screen |
| Notes | This test is currently unaccredited due to a temporary change of assay following a recent reagent supply issue. |