FISH analysis for Chimerism analysis (XX/XY) Post-BMT
|Category||Specialist Integrated Haematological Malignancy Diagnostic Service (SIHMDS) >> Cytogenetics|
FISH involves the application of fluorescent DNA probes specific to genes or genetic regions of interest that highlight abnormalities involving these regions.
In patients who have received sex-mismatched bone marrow transplants, X/Y FISH is a useful means of rapidly screening large numbers of bone marrow cells for the presence of host cells that may be a sign of engraftment failure or early relapse.
|Sample & container required||See notes section|
|Sample volume||Samples would not be rejected on the basis of small volume, however, 5 mL is ideal.|
|Turnaround time||Post treatment FISH analysis is treated as routine; 95% should be reported within 21 calendar days according to national guidelines. Please note however that the average turn around for X/Y FISH in our laboratory is approximately 4 days.|