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FISH analysis for Chronic Lymphocytic Leukaemia / small lymphocytic leukaemia

Category Specialist Integrated Haematological Malignancy Diagnostic Service (SIHMDS) >> Cytogenetics
Test background

FISH involves the application of fluorescent DNA probes specific to genes or genetic regions of interest that highlight abnormalities involving these regions.

Clinicial Indications

In CLL/SLL, a panel of FISH probes is applied at diagnosis to identify common genetic abnormalities that are known to be strongly associated with prognosis in this disease and may assist in devising the most suitable management strategy.

The CLL FISH panel includes the following probes:

13q14(DLEU)/13qter
TP53  17p13
ATM 11q22

Chromosome 12 centromere

In cases of uncertain diagnosis, a probe for IGH may also be applied if it is deemed appropriate.

It may be appropriate to repeat the CLL FISH panel after treatment to assess response or to investigate disease progression.

Reference range

N/A

Sample & container required See notes section
Sample volume Samples would not be rejected on the basis of small volume, however, 5 mL is ideal.
Turnaround time FISH analysis in CLL/SLL is treated as routine; 95% should be reported within 21 calendar days according to national guidelines. Please note however that the average turn around for CLL FISH in our laboratory is approximately 9 days.
Notes

Sample required:

Bone marrow or peripheral blood in cytogenetic transport medium, lithium heparin or EDTA. Samples which are non-sterile, clotted or collected in sodium citrate, fixative or saline are not suitable.

To ensure appropriate analysis and interpretation it is important to provide clear and concise clinical information.