FISH analysis for T lymphobastic leukaemia/lymphoma
| Category | Specialist Integrated Haematological Malignancy Diagnostic Service (SIHMDS) >> Cytogenetics |
|---|---|
| Test background |
FISH involves the application of fluorescent DNA probes specific to genes or genetic regions of interest that highlight abnormalities involving these regions. |
| Clinical Indications |
Conventional cytogenetic analysis is carried out routinely on diagnostic T-ALL samples. If a normal karyotype is found, further FISH is applied using a panel of probes for common recurrent abnormalities in this disease, comprising: TR A@ |
| Reference range | N/A |
| Sample & container required | A minimum of 2-3mls Bone marrow in lithium heparin. 3-5mls peripheral blood in lithium heparin (if disease cells are present in sufficient numbers to allow cell culture and/or FISH studies, as appropriate) EDTA PB and BM samples (>1ml) are acceptable for FISH only studies as appropriate. Please note the laboratory does not provide transport medium. Samples sent in transport media from an external laboratory containing Lithium heparin will be accepted if no other media available |
| Sample volume | Samples would not be rejected on the basis of small volume, however, 5 mL is ideal. |
| Turnaround time | Rapid test by FISH: 95% should be reported within 3 calendar days. Urgent Karyotype/FISH: 95% should be reported within 14 calendar days. Routine referrals (follow-up) 95% should be reported within 21 calendar days. |
| Notes | If the blood counts are abnormal (high or low white cell count) the volumes of BM/PB requested can be adjusted accordingly. For culture (karyotyping) a WCC of 5×10^6 cells per ml is optimal. Delayed samples: |