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HLA typing -PCR-SSO (low/medium resolution)

Category Immunology >> Histocompatibility and Immunogenetics
Test background

HLA typing at medium/low resolution typing for HLA-DQA1, -DQB1, -DRB345 is performed using PCR-SSO. This technique is primarily used for the HLA typing of solid organ patients and donors.

Clinical Indications

HLA typing at HLA-DQA1, -DQB1, -DRB345 alongside high resolution NGS HLA typing allows for a full HLA typing result to be achieved for solid organ patients and donors. HLA typing alongside HLA antibody detection techniques allows full HLA compatibility to be determined for solid organ patients and their prospective donors.

Reference range

An HLA tissue typing report will be provided.

Sample volume Minimum - 4 mL
Sample container 4mL Whole Blood – EDTA (lavender top)
Transport storage Samples must be sent by courier.
Turnaround time Renal/ pancreas patients and donors -30 days

Samples must be sent via first class post or courier.

The laboratory is accredited for this test by the European Federation of Immunogenetics (EFI) and UKAS for HLA typing using EDTA samples.