Liver autoantibodies immunoblot
| Category | Immunology |
|---|---|
| Test background |
A liver immunoblot is performed when staining suggestive of mitochondrial, LKM or LC-1 antibodies are detected on LKS IIF or when liver associated ANA patterns are detected on Hep2 IIF to confirm specificity. Additionally this method is the only way to reliably detect SLA antibodies and should also be performed when there is a high suspicion of autoimmune hepatitis but initial LKS and ANA Hep2 screening are negative. The immunoblot comprises antigens for M2, 3E(BPO), SP100, PML and GP210 antibodies, which can be found in primary biliary cholangitis, and also LKM type 1 and SLA antibodies, which can be seen in autoimmune hepatitis type. |
| Clinical Indications |
Diagnosis of primary biliary cholangitis (PBC), Autoimmune hepatitis type (AIH) |
| Reference range | Negative |
| Sample & container required | Serum (RST rust top) |
| Sample volume | 5-10 mL blood (1 mL serum) |
| Turnaround time | 14 days |