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Lymphocyte function

Category Immunology
Test background

This assay assesses T cell responses to a panel of mitogens and recall antigens. Profound loss of response to the lectin phytohaemagglutinin (PHA) is seen in severe combined immunodeficiency (SCID) and some combined immune deficiency (CID). The assay is performed on whole blood or separated PBMC (peripheral blood mononuclear cells) if T cell counts are low.

This assay measures the incorporation of radioactive tritiated thymidine into the DNA of proliferating cells following activation by mitogens or recall antigens.

Clinical Indications

Suspected combined immunodeficiency

Reference range

See report

Sample & container required 10-20 mL heparin (green top) for adults; 5-10 mL heparin (green top) for children; Do not separate or refrigerate; Test within 24 hours.

Please note an unrelated healthy control blood sample MUST be sent with patient sample.

Sample volume See above
Turnaround time 10 days

Test must be pre-arranged with Laboratory on ext. 10130. Not performed for HIV positive patients. Not currently performed in house; this test is referred to the Camelia Botnar Laboratories at Great Ormond Street Hospital, London.

Samples must be <24 hours old on receipt and can only be accepted Monday to Thursday (by 4pm).