Skip to main content

Login to North West London Pathology – consumables service

If you need a new account or you have a query about your order, please telephone the pathology call centre on 0203 313 5353.

If you have forgotten your password you can reset this yourself. Please click here if you have forgotten your password.

Results Line and Enquiries

0203 313 5353


Category Biochemistry
Test background

Methotrexate is a competitive inhibitor of dihydrofolate reductase, an enzyme responsible for tetrahydrofolate (THF) regeneration. 5,10-methylenetetrahydrofolate, formed from THF, is essential for the de novo synthesis of the nucleotide thymidine, required for DNA synthesis. Methotrexate therefore inhibits the synthesis of DNA, RNA, thymidylates and proteins, and is used as an anti-cancer treatment.

Clinical Indications

Patients on high dose methotrexate infusion.

Reference range

See report

Sample & container required SST (gold top), serum (red top), or EDTA (lavender top)
Sample volume 2 mL
Turnaround time Up to 6 weeks

Not currently performed in house; this is a referred test.
Please state the date and time of the last dose of methotrexate.