MPN panel (next generation sequencing)
| Category | Specialist Integrated Haematological Malignancy Diagnostic Service (SIHMDS) >> Molecular Pathology |
|---|---|
| Test background |
The MPN NGS panel is comprised of 15 genes to cover the most relevant targets in myeloproliferative neoplasms. This assay comprises of 82 amplicons in 15 genes frequently mutated in myeloid malignancies. The regions covered by this panel are: ASXL1 (exon 13); CALR (exon 9); CBL (exons 8 and 9); CSF3R (exons 14-18); EZH2 (exons 2-20); IDH1 (exon 4); IDH2 (exon 4); JAK2 (exons 12 and 14); MPL (exons 10); SETBP1 (exon 4 partial); SF3B1 (exons 13-16), U2AF1 (exons 2 and 6); NRAS (exons 2 and 3), KRAS (exons 2-4); SRSF2 (exon 1). |
| Clinical Indications |
Investigation of suspected (philadelphia chromosome negative) myeloproliferative neoplasms polycythemia vera, essential thrombocythemia and myelofibrosis. |
| Sample & container required | 4ml peripheral blood or >1ml bone marrow in EDTA. For other sample types, please liaise with the laboratory. |
| Turnaround time | 21 calendar days |