Ovarian antibodies
| Category | Immunology |
|---|---|
| Test background |
Antibodies targeting the steroid producing cells of the adrenal cortex, ovaries and testes are found in around 80% of patients with Addison’s disease and are also seen in Autoimmune Polyendocrine Syndrome (APS) type I (APECED) and APS type II. Around 10% of women with Addison’s disease also have premature ovarian insufficiency. The antibodies are directed against three p450 cytochrome enzymes; 21α-hydroxylase (P450c21), found in adrenal steroid producing cells but absent from testes leydig cells and ovarian theca interna, 17α-hydroxylase (P450c17) and cholesterol desmolase (P450scc). Positive antibodies against ovary parenchyma are seen in 15-50 % of patients with premature ovarian failure. This test is performed by indirect immunofluorescence on primate triple block (adrenal/testes/ovary) tissue sections. |
| Clinical Indications |
Suspected premature ovarian failure |
| Reference range | Negative |
| Sample & container required | Serum (RST rust top) |
| Sample volume | 5-10 mL blood (1 mL serum) |
| Turnaround time | 14 days |