Total Procollagen 1 N-Terminal Propeptide (P1NP)
| Category | Biochemistry >> Bone Metabolism |
|---|---|
| Test background |
During the renewal of the skeleton, type 1 procollagen's amino- and carboxy-terminal regions are cleaved by specific proteases during its conversion to collagen and subsequent incorporation into bone matrix. The presence of these N-terminal extension propeptides, known as type 1 procollagen amino terminal peptides (P1NP), therefore reflects the deposition of collagen and can be defined as a marker of bone formation. P1NP is released into the blood stream during type 1 collagen formation. Elevated levels are found in patients with increased bone turnover. As a bone marker, it may be use in the assesment of bone turnvoer in metabolic bone disease and monitoring of therapy and is specifically used for monitoring of bone formation during anabolic therapy. |
| Clinical Indications |
Assessing treatment response and compliance to osteoporosis therapy (such as bisphosphonates), but specifically the response to treatment by human PTH (Teriparatide). |
| Reference range | Pre-menopausal women: 15.1-58.6 ng/mL. |
| Sample & container required | See Sample Collection. SST (gold top). Samples can be stored at 4 ºC for up to 1 week, or at -20 ºC for longer storage (other non-accredited but acceptable sample types include, EDTA (lavender top) or heparin (green top) tubes). |
| Sample volume | 250 µL |
| Sample collection | Time of collection: if monitoring response to treatment, samples should be collected at the same time of day e.g. always a morning clinic, or always an afternoon clinic. Ideally a morning fasting sample should be collected . Serial measurements on the same patient are recommended before therapy and at 3 and 6 months after therapy (and at subsequent 3, 6 or 12 monthly intervals as required). |
| Transport storage | Stable at 2-8°C for 1 week. Please freeze pending dispatch for analysis. Sample can be sent by first class post. |
| Turnaround time | 4 weeks |
| Notes | If monitoring the response to treatment, the sample should be collected at the same time of day. Grossly haemolysed samples are unsuitable for this assay. |